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An English football fan in the Sporting end, wearing the Portuguese club's kit, sang 'Tottenham get battered everywhere they go,' at the travelling Spurs fans, as you can see in the video below.
Spurs had a very welcoming return to the Champions League last week, when Richarlison's late brace earned them a win against Ligue 1 Marseille.
The Brazilian was very emotional as his two goals were on European debut, and made sure his side got off to the perfect start in the group.
However, just six days later, and things weren't looking quite a rosey for Antonio Conte's side, as they were on the wrong end of a 2-0 defeat by Sporting.
The chant by the Sporting 'fan' was popularised by supporters of Spurs' rivals West Ham United and Arsenal and became quite the sensation last year.
The song could be heard almost constantly during last winter's PDC World Darts Championship, that was when the fans weren't booing anyone not from England, specifically Scottish players.
Spurs were also trolled by Thierry Henry, with the former Arsenal striker's punditry for Sporting's second goal going viral for all the wrong reasons for the north London side.
It wouldn't have helped Conte's team on Tuesday that Tottenham academy product Marcus Edwards produced arguably the performance of the night, as the hosts scored two late goals.
The 23-year-old was on such impressive form that some were even calling for him to be given a chance by Gareth Southgate in the England national team.
Asked on commentary if he thought Edwards was in line for a call up, former Three Lions' midfielder Owen Hargreaves said, "How can he not when he's playing like that?
"There were loads of internationals and he was the best player on the pitch. That's what you want as a player, an opportunity. He can play in different positions.
"He's playing as a false nine really well and can play as a number 10. He just takes the ball so well. I think there's always a space for a player like that in the team."
Southgate will call up his latest squad this week, which will be the last one before the World Cup, and Edwards would be unlikely be in that one, especially as Manchester United pair Marcus Rashford and Jadon Sancho are expected to be recalled.
Still, at such a young age, the former Vitoria player still has plenty of time to push his cause for a future spot in an England squad.