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The Three Lions beat Germany in a knockout tournament for the first time in 55 years after goals from Raheem Sterling and Harry Kane sealed a huge round of 16 win at Wembley on Tuesday.
And there could be a first tournament start for Jadon Sancho, who has struggled for game-time at Euro 2020 thus far.
:newspaper2: Gareth Southgate is set to move to a more attacking back four formation for #ENG's Saturday night quarter-final against #UKR, and as Bukayo Saka has a "slight knock", Jadon Sancho could be in line for his first start of #EURO2020 [@TeleFootball] pic.twitter.com/bDDn603nB6- UtdDistrict (@UtdDistrict) July 2, 2021
The newly-confirmed Manchester United winger managed just six minutes of action in the win over Czech Republic but is in contention to play from the off at the Stadio Olimpico following an injury to Arsenal's Bukayo Saka.
Saka has started England's last two games but has picked up a knock and is therefore a doubt. Mason Mount, meanwhile, is in line to return to the fold after finishing his period of isolation.
Scorers Sterling and Kane will keep their places, as will the likes of Luke Shaw, Kyle Walker and John Stones.
Harry Maguire, Declan Rice and Kalvin Phillips are all a booking away from suspension but the trio are expected to start despite Jose Mourinho's suggestion that Southgate rest all three.
"I was on a yellow before the semi-final with Germany at Euro 96 and would have missed the final had we ever got there, had I picked up a yellow in the semi," Southgate said in a press conference.
"You have to have the same commitment because if you don't we are not going to get to the semi-final.
"You still have to be committed, avoid unnecessary yellows if you can but accept you might mistime something."
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