During the group stages, the Dutch impressively swept aside Ukraine, Austria and North Macedonia in Group C - with Frank de Boer's side being tipped as dark horses to win the contest by many fans and pundits.
The Czechs naturally play counter-attacking style football which can prove a nightmare for possession-based opponents such as the Netherlands.
However, the Dutch have already scored some quick breakaway goals themselves, so it will come as no surprise if this evening's game is a cagey affair.
4 great games coming your way on @ITV! :tv:
5PM - #NED vs #CZE
8PM - #BEL vs #POR
5PM - #CRO vs #ESP
8PM - #FRA vs #SUI#ITVFootball ' #Euro2020 pic.twitter.com/4zdlSSLNDQ
- ITV Football (@itvfootball) June 25, 2021
Unbelievably, the Dutch are without a win in a Euros knockout stage since 2004 - but they look to have what it takes to buck that trend this summer.
With the knockout stages now underway, our tipster Robert Mann takes a look at some of the best bets from Netherlands vs Czech Republic to get your teeth stuck into.
Netherlands vs Czech Republic betting tips & predictions
Netherlands are 4/6 to win, with Czech Republic priced at 4/1 to come away with the victory. A draw is priced at 14/5.
Prediction: 2-1 (7/1)
Half-Time/Full-Time: Draw/Netherlands (7/2)
Frank De Boer's side are unbeaten in their last seven matches, too, and they should have too much class in the final third for the Czechs this evening.
We're predicting the teams will head into the break level-pegging before the Dutch come out all guns blazing in the second half, before sinking their teeth into the jugular of the Czechs.
First Goalscorer: Memphis Depay (16/5)
Last week, we tipped Memphis Depay to score first against North Macedonia - and he did just that.
Depay has been directly involved in 13 goals in his last ten appearances for his country, scoring nine times and picking up four assists.
We reckon odds of 16/5 for Depay to score first tonight is, surely, too juicy of a price to miss out on.
Price Boost: Netherlands to win and both teams to score (3/1)
The Dutch appear to have real momentum and carry a huge goal threat after eight goals in their three group games.
Having said that, though, they are not exactly watertight at the back - and we think the Czechs have a good chance of getting at least one goal on the board.
And if both teams do score, the Netherlands should have enough class on the night to be able to see the game out by the final whistle.
Odds are correct at the time of publication and are, as always, subject to change.
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Netherlands team news
Luuk de Jong, who has only played ten minutes for the Netherlands during Euro 2020, will not feature again in the tournament after sustaining a knee injury.
Meanwhile, Marten de Roon should return to the starting line-up after being rested last time out.
It's coming...:ocean:#EURO2020 #NEDCZE pic.twitter.com/nNbBsEzBkS
- OnsOranje (@OnsOranje) June 25, 2021
Czech Republic team news
The Czech Republic will be forced into a change at left-back after Jan Boril picked up his second booking of the tournament against England.
Czech talisman Patrick Schick will lead the line despite struggling with a facial injury he picked up in the game against Croatia at Hampden Park.