The Scots will travel to Wembley - the home of English football - knowing that their 23-year wait to qualify for a major tournament could be on the brink of collapse.
The Three Lions will be full of confidence after that opening game win and will, almost certainly, fancy their chances of piling more misery on their old rivals.
Scotland have taken the game to England in recent years, though, and are not to be underestimated, by any means.
Realistically, Scotland need a result in London to stand a chance of qualifying for the knockout stages - but they face stiff opposition against one of the competition favourites.
With this summer's tournament now in full swing, our tipster Robert Mann takes a look at some of the best bets from England vs Scotland to get your teeth stuck into.
England are 3/10 to win, with Scotland priced at 19/2 to come away with the victory. A draw is priced at 4/1.
Prediction: 3-0 (7/1)
Half-Time/Full-Time: Draw/England (3/1)
Given this is the oldest fixture in international football, and a game between two of the world's fiercest rivals, expect plenty of fireworks.
The last time the two teams met at Wembley, England came out 3-0 winners, and we are predicting the same scoreline again tonight.
If England's previous games are anything to go by, Gareth Southgate's men should put the pressure on Scotland from the off, breaking them down with every attack.
After tirelessly breaking the Scots down for the first 45 minutes, we're predicting the Three Lions to turn up the gas in the second half and bag the three points.
Player to assist: Kalvin Phillips to assist 1+ goals (21/10)
Kalvin Phillips, who deservedly won man of the match against Croatia, looks like an ever-improving player for both club and country.
The Leeds United midfielder put in a standout performance as England beat their Group D rivals 1-0 at Wembley on Sunday.
Phillips laid on the only goal of the game for Raheem Sterling to cap off a fine individual display.
While UEFA handed its star of the match award to Sterling, Phillips would claim the official England man of the match gong.
We reckon his fine form will continue against Scotland and another assist could very well be on the way.
First goalscorer: Harry Kane (9/4)
All of England's play is garnered towards providing for their main forward - and we think Harry Kane should get off the mark against Scotland.
Barring injury, Kane is almost guaranteed goals and he sounds like a given to score first against a shaky Scotland defence on Friday night.
Odds are correct at the time of publication and are, as always, subject to change.
Please gamble responsibly. For more information visit: https://www.begambleaware.org.
Gareth Southgate should reward Kalvin Phillips and Kieran Trippier with starts after impressive displays in the opener against Croatia.
After rehabilitating from an ankle injury, Harry Maguire has claimed he is fit enough to start - but Southgate will have to remain cautious about including him so soon.
Hard at it in the heat :runner:♂️:sunny: pic.twitter.com/fRXiy53yIV
- England (@England) June 16, 2021
Steve Clarke will be hopeful that Kieran Tierney's calf injury clears up after he missed the opening game against the Czech Republic.
Skipper Andy Robertson put in a stellar performance that day - and he will be key to Scotland's success from crosses and set pieces.
Gutted. Stay positive :flag_black: pic.twitter.com/Y0pyJXc8lF
- Andy Robertson (@andrewrobertso5) June 14, 2021
Other than that, Clarke has a fully fit side to choose from - but he will need to answer his critics with a fresh lineup after pundits slammed his starting 11 against the Czechs.
Chosen for YouChosen for You
Most Read StoriesMost Read