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The Three Lions boss named his final 26-man squad for the European Championships on Tuesday, with Trent Alexander-Arnold making the cut and Jesse Lingard, James Ward-Prowse and Ollie Watkins all missing out.
In tonight's game, fresh talents in Jude Bellingham and Jack Grealish could both start and will hope to impress Southgate ahead of England's opening match of the tournament against Croatia on Sunday, 13 June.
Instead, the Three Lions will have a makeshift look about them in their penultimate game before the tournament.
Here's everything you need to know ahead of the game.
The game will be shown live on ITV 1 and can be streamed online via the ITV Hub.
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