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The eagerly anticipated bout will be the first boxing match in a stadium in the UK since the COVID-19 pandemic broke out in March 2020 - and there promises to be fireworks.
In a match-up of two former Olympic gold medalists from London 2012, Joshua will go toe-to-toe in the ring with the Ukrainian - the WBO mandatory challenger - in just two months time.
If this trailer doesn't give you goosebumps, then we don't know what will.
Joshua was expected to meet Tyson Fury to unite the heavyweight titles this summer, but the 'Gypsy King' was ordered by a court to take on 'Bronze Bomber' Deontay Wilder in their trilogy clash instead.
AJ will now face Usyk, though, who is unbeaten in his 18 professional bouts to date - posing a threat to Joshua's stellar legacy.
The Ukrainian will pose a hugely tough test for Joshua who must win to keep his hopes of claiming all the heavyweight belts alive, but how will he fare?
Here's what we know about their fight so far.
This 12-round heavyweight title fight will take place on Saturday, September 25.
The clash will be for Joshua's WBA (Super), IBF, WBO, and IBO heavyweight titles
As London is the host city of this clash, UK fans can expect the main event to get underway at around 10:30pm.
More timings and an undercard are to follow.
The fight will be held at the Tottenham Hotspur Stadium in front of 60,000 fans later this year.
The stadium in north London has a capacity of over 60,000, however, vaccination proof may be required to attend sports events of about 20,000 or more capacity by the end of September.
Unified Heavyweight Champion of the World @AnthonyJoshua will put his IBF, WBA, WBO and IBO World Titles on the line against Oleksandr Usyk at Tottenham Hotspur Stadium on Saturday, September 25. #DestinationTottenham #JoshuaUsyk- Tottenham Hotspur (@SpursOfficial) July 20, 2021
The fight will be shown on Sky Sports Box Office and details on their coverage will soon follow.
AJ vs Usyk @ Tottenham :stadium::muscle:@anthonyjoshua will put his world title belts on the line against @usykaa at the @SpursOfficial Stadium :flag_gb::flag_ua:- Sky Sports Boxing (@SkySportsBoxing) July 20, 2021
:point_right:Saturday September 25, live on Sky Sports Box Office:movie_camera: pic.twitter.com/igCf1F9opl
Anthony Joshua is 4/11 is the heavy favourite to win the fight, while Oleksandr Usyk is the marginal underdog at 2/1. A draw is priced at 25/1. Expect odds to change heavily leading up to fight night.
Odds are correct at the time of publication and are, as always, subject to change.
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